Retention times shift out in acquity uplc h class wkb76868. Waters acquity uplc overview and maintenance manual pdf. Sample manager flow through needle plus smftn plus and the. Location of the sample manager s purgesolvent degasser degasses the sample manager.
If passive recovery of the sample compartment temperature was not successful, perform an active defrost. Page 19 the system includes a solvent manager, sample manager, column heater, detector. Acquity uplc hclass system guide waters knowledge base. What is the syringe draw rate for automatic in waters uplc h class system. This waters acquity uplc h class sample manager ftn is in excellent condition. In the data application, in the control panel of the sample manager, right click and then. After replacing the inject valve and sample loop it will be necessary to prime the wash syringe. Waters acquity uhplc hclass with qda system conquer. For others benefit this can be found in the acquity console under the sample manager ftn maintenance pull down menu the choose calibrate z axis. Waters acquity uplc hclass quaternary solvent manager. For other locations worldwide, phone and fax numbers appear in the waters web site. The acquity uplc hclass systems sample manager features a. Location of the sample manager s purgesolvent degasser degasses the sample manager s purge solvent.
The waters acquity uplc system is intended for analyzing compounds and introducing separated samples into a mass spectrometer. View and download waters acquity uplc overview and maintenance manual online. Waters acquity uplc hclass series system manual pdf download. If the sample manager is powered off for any reason, you must reboot the sample organizer, and then power on the sample manager. The familiar design of the acquity uplc h class s quaternary solvent manager qsm and sample manager smftn, with flowthrough needle design, gives you all the flexibility and usability of your current hplc while still achieving the highly efficient separations that only uplc can provide. Waters acquity uplc hclass sample manager ftn conquer. Acquity uplc, millennium, uplc, and waters are registered trademarks. System components instrument modulesthe acquity uplc h class core system includes a quaternary solvent manager, sample manager flow through needle, column heater, detectors tunable ultraviolet, photodiode array, evaporative light scattering, fluorescent, or mass spectrometry, and an acquity uplc column. This waters acquity uplc h class system is the only quaternary based liquid chromatographic system that was designed for true uplc performance without compromise. Acquity uplc h class sample manager see how the acquity uplc h class flowthroughneedle sample manager enables familiar hplc operation and uplc performance. The fl has a slightly lower total system volume 95 ul versus 100 ul for the ftn. Sample manager ftn replacing the needle, guide, and vespel seat this video describes how to replace the sample needle, needle guide, and vespel seat in the acquity h class sample manager flow through needle. Whether you routinely develop new methods or you perform routine analysis and support large numbers of samples, the acquity uplc hclass system can help you reach your laboratorys goals.
Intended use of the acquity uplc h class system the waters acquity uplc h class system is for research use only. Calibrating to calibrate lc systems, follow acceptable calibration methods using at least five standards to generate a standard curve. Waters acquity system manual pdf download manualslib. Feb 20, 2021 be aware of tolerances and derating due to elevated room temperature. Acquity uplc sample manager fl fixed loop rotary tray not calibrated message. Acquity uplc sample manager problem chromatography forum. Open the empower pro and click on the run sample button. This waters acquity uhplc hclass system is the only quaternary based liquid chromatographic system that was designed for true uplc performance without compromise. Acquity uplc hclass system guide conquer scientific.
Waters acquity uplc hclass system with sample manager ftn. Class b products are suitable for use in both commercial and. All msgrade solvents used with the acquity uplc system should be properly. This option can be found in the sample manager under the control menu.
In the sample manager information window, click control reset sm, to reset the sample manager. Waters acquity uplc operators, overview and maintenance manual. Sample manager ftn replacing the needle, guide, and vespel seat this video describes how to replace the sample needle, needle guide, and vespel seat in the acquity h class sample manager. This video describes how to replace the sample needle, needle guide, and vespel seat in the acquity h class sample manager flow through needle. It gives an overview of the systems technology and operation. Laboratory equipment waters acquity uplc h class series system manual. View and download waters acquity isocratic solvent manager overview and maintenance manual online. Waters acquity the acquity uplc system eliminates significant time and cost per sample from your analytical process while improving the quality of your results. Acquity uplc june 2011 driver packdp3 part number 667004296 driver pack 4 part number 667004903. In the acquity uplc console, select sample manager from the system tree. View and download waters acquity cm30s overview and maintenance manual online. Acquity uplc hclass system from waters corporation. Two columns, as standard maximum length of 150 mm with filter or guard column or four columns maximum length of 50 mm can be supported with optional tubing kit, up to 4. The h class sample manager needlewash is in contact with the external surface of the needle and des not have to match the mobile phase, it only has to ensure the sample solubility.
The i class fl is configured with a fixed loop injector like the original acquity system, but with a lot of improvements. For exisitng and transfered methods, what has worked in the past is a good place to start. Waters acquity isocratic solvent manager overview and. Acquity uplc system acquity uplc h class system acquity uplc h class bio system acquity uplc i class system acquity uplc systems with 2d technology dp4sr1 updates and supersedes these previously released driver packs.
View and download waters acquity system manual online. Acquity acquity uplc hclass sample manager ftn hclass condition. The column manager can regulate the temperature of columns from 4 to 90 c. Acquity cm30s laboratory equipment pdf manual download. Waters acquity uplc hclasshplc familiarity with uplc performance. Any customer who purchases waters products are advised that in order to hold a valid license to use the waters. Plumbing the binary solvent manager and sample manager. Mobile phase degasser chamber 4 removes dissolved gasses from mobilephase solvents and exhausts them, along with any condensates, through waste tubing.
Pdf acquity uplc hclass system guide chetan jagdale. The waters acquity uplc h class chromatographic system features the quaternary solvent manager and autoblend technology, which enables you to automatically b. The new h class is designed to replace hplc systems and accelerate uplc adoption among hplc users milford, mass. Intended use of the acquity uplc hclass system the waters acquity uplc hclass system is for research use only. Specify the project and a new window run sample appears.
Used waters acquity with pda system in pleasanton, ca. The waters acquity uplc h class plus system delivers the flexibility of quaternary solvent blending with the advanced performance of uplc separations. Acquity hclass smftn maintain test procedures waters. Waters designed the acquity uplc system to isolate, concentrate. Acquity uplc hclass system guide waters corporation.
The system s holistic design is targeted for routine analysis and method development use and is perfe\ ctly suited for running both hplc and uplc applications while still realizing the improved. Waters acquity uplc h class system guide 2 mb waters acquity uplc h class system instrument specifications 347 kb waters acquity uplc sample manager overview and maintenance guide 4 mb waters acquity uplc system instrument specifications 901 kb waters acquity uplc system operators guide 5 mb. The ftn is a flow through needle injector design, like the one in the acquity h class system, but again with improvements. In the acquity uplc console, select sample manager ftn from the system tree. Before you prepare the smfl for operation, prepare the solvent manager. The acquity uplc hclass column manager is an option for helping to. The acquity uplc h class system combines the speed and performance of uplc with the ability to run hplc separations. If you detached the connector from the front of the instrument, reattach it, and poweron the sample manager. Waters acquity uplc hclass series system manual pdf.
This morning i did an injection on vial a8 well plate 48 and i saw the injector going to b8 instead. Whether you routinely develop new methods or you perform routine analysis and support large numbers of samples, the acquity uplc h class system can help you reach your laboratorys goals of decreasing the time to result, while. See how the acquity uplc hclass flowthroughneedle sample manager enables familiar hplc operation and uplc performance. Acquity uplc hclass quaternary solvent manager youtube.
Before you prepare the smftn for operation, prepare the solvent manager. Receives solvent from the primary pump and delivers it to the vent valve. Available with 30 day warranty coverage, and 90 day with onsite installation. Poweron the sample manager by pressing the power switch on the top, lefthand side of the door. Answered 40 views 2 comments 0 points most recent by dejian june 2020. Demonstrates the correct procedure for removing the needle assembly in the acquity uplc sample manager. Waters acquity uplc hclass system conquer scientific.
For instructions on preparing the solvent manager, see acquity uplc quaternary solvent manager operators overview and maintenance. Waters acquity arc system system manual pdf download manualslib. In contrast to the classical uplc system sample manager where we have 3 different injection modes, and where two of them are using an overfill factor, the ftn sample manager is only drawing the amount of sample you specify in the sample set, so no sample waste. If you need additional help, contact waters for onsite service. Some reagents and samples used with waters instruments and devices can pose chemical. Acquity uplc sample organizer operators overview and. Waters acquity uplc the acquity uplc system eliminates significant time and cost per sample from your analytical process while improving the quality of your results. See how the acquity uplc h class flowthroughneedle sample manager enables familiar hplc operation and uplc performance. Acquity uplc system operators guide waters corporation. Acquity uplc sample manager fl fixed loop rotary tray not calibrated message answered 40 views 2 comments 0 points most recent by dejian june 2020 run not advancing to the next injection. Licenses for waters software products are not transferable. Waters contact information contacting medium information internet the waters web site includes contact information for waters locations worldwide.
The waters acquity uplc hclass system is for research use only. Oct 17, 2019 acquity hclass sample manager ftn does not cool below 9 degrees c wkb2716 last updated. When setting a 4 c sample temperature at 26 c ambient, a healthy h class is specified to achieve an actual sample compartment temperature between 5 c and 11 c. When you develop methods, follow the protocol for the adoption of analytical. From the system view of the acquity uplc console, select the sample manager, and then click control prime. Wat today introduced the new waters r acquity uplcr h class system incorporating the proven, robust and reliable performance of acquity ultraperformance lcr uplc with the operational familiarity of. Whether you routinely develop new methods or you perform routine analysis and support large numbers of samples, the acquity uplc h class system can help you reach your laboratorys goals of decreasing the time to. We have 1 waters acquity arc system manual available for free pdf download. Class b products are suitable for use in both commercial and residential. Waters designed the acquity uplc system to isolate, concentrate, separate, detect, and measure individual analytes in solution mixtures for research applications.
Two columns, as standard maximum length of 150 mm with filter or guard column or four columns maximum length of 50 mm can be supported with optional tubing kit. Demonstrates how to replace the sample loop in the acquity uplc sample manager. Demonstrates how to calibrate the needle z axis in the acquity uplc sample manager. Waters acquity h class sample manager ftn waters acquity h class quaternary solvent manager computer and software cables and connectors note. For instructions on preparing the solvent manager, see acquity uplc binary solvent manager operators overview and maintenance information. Replacing the needle, guide, and vespel seat waters videos. Symbol definition authorized representative of the european community confirms that a manufactured product complies.
High pressure on acquity hclass qsm wkb21450 waters. The waters acquity uplc iclass plus systems holistic design is. You will need to remove the well plate and using the z buttons slowly bring the needle down very close to touching the tray bottom, then press save. It is the core of the patrol uplc process analysis system, and can be added to any existing acquity uplc, acquity uplc h class, or acquity uplc h class bio system. Control the sample compartment to 30c and wait until the sample temperature 20 degrees c. The acquity uplc psm automates your online and atline inprocess analyses. Acquity uplc i class sample manager flow through needle smftn cause drawing sample rate excessive can be caused by the syringe draw rate being too fast, possibly because the solution is viscous. Waters acquity h class column manager uplc column manager.
Thumbscrew in notched area sample manager or cms collars flange wide portion of upper cutout. When sample temperature reaches room temperature, the engines heat sink should be fully defrosted and ready to resume operation. This procedure must be performed after replacing the sample needle. Acquity uplc system operators guide citrine solutions. Acquity uplc hclass system from waters corporation product. Acquity uplc hclass plus system instrument specifications. If, after you cycle power to the detector, the led remains red, report the problem to waters technical service. By outperforming traditional or optimized hplc, the system allows chromatographers to work at higher efficiencies with a much wider range of linear velocities, flow rates, and.
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